Cancer Organoid Culture Kit
A set of NCP, dispersion- tool/enzyme, and a medium supplement for cancer organoid culture
In traditional 2D cell culture, cells are grown on flat dishes of polystyrene plastic that is very stiff and unnatural and form monolayer. Primary cancer cell culture is inefficient and atypical under that condition. Because stroma cells which mainly include fibroblast showing a high proliferative activity responsive to stimulation of the cell adherence, and those cells strongly proliferate more than cancer cells. The dominant proliferation of fibroblast is constricted in 3D cell culture. Therefore, collagen-gel-embedding culture is already used in clinic.
ORGANOGENIX’s Cancer Organoid Culture Kit is suitable for primary culture of cancer cell from specimen, and it includes NanoCulture Plate 96-well, dispersion solution, mesh, and special medium.
This product is commercialized from accomplishments of collaboration with National Cancer Research Center (Kashiwa, Japan).
The product’s features:
1) Shorter culture period compared to collagen-gel-embedding culture
2) No gel containing component that lead to a easy medium exchanging / handling
3) Multi-well format leads to easy conduction of anti-cancer drug test
|Cancer Organoid Culture Kit||PCK-5b||for 5 tissue
|Cancer Organoid Culture Medium P1 type||NCM-P1b||100ml||-20℃|
Schematic diagram of primary cancer cell culture in 3D
Tumor specimen is minced, treated with dispersion solution, and filtrated by mesh. Obtained cells are seeded in to NCP with special medium. One day after, change 50% medium, and repeat the medium change every 2-3 days.
Images of primary cancer cell spheroids
After 7 days culture, primary cancer cell spheroids are confirmed (arrow head). These spheroids include cancer cells and stroma cells, i.e. “co-culture”.
Example data: Gefitinib sensitivity test of primary cancer cell spheroid
Gefitinib sensitivity test of primary lung cancer was conducted. Cells having EGF exon 19 del. (sample C and D) showed a higher sensitivity to Gefitinib compared with cells having EGF WT (sample A and B) in this 3D culture system.