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Spheroid Dispersion Solution, Lysis Buffer

【Spheroid Dispersion Solution (SD4X)】

Spheroid Dispersion Solution (SD4X) enzymatically disperse spheroids to single cells. The resulted single cells can be used for cell counting, FACS and subculture, etc.

Product Name Cat.# Qty Storage Document
Spheroid Dispersion Solution(4X) SD4X 15ml -20℃ Handbook

MSDS

Procedure

NCM-M-1

1. Collect spheroids by pipetting and transfer into the tube
2. Add SD4X and incubate for 5 – 10 min at 37℃
3. Completely disperse to single cells by pipetting

【Spheroid Lysis Buffer (SLB)】

This reagent can easily lyse the spheroid on NCP. The spheroid lysate is suitable for viability assay based on DNA quantification using PicoGreen. This reagent is not recommended for protein extraction because it contains SDS.

Product Name Cat.# Qty Storage Document
Spheroid Lysis Buffer SLB 7.5ml x 2 Room Temp. Handbook

MSDS

Procedure

NCM-M-1

1. Add SLB (1/10 aliquots of culture medium)
2. Incubate for 5 min at RT
3. Mix by pipetting until homogenous solution