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Drug screening under 3D cell culture condition

For customer who wants to explore candidate compounds having anti-cancer effect

We conduct screening from your library (chemical compound, antibody, siRNA etc.) with your preferable 3D cell culture method. We can do large scale screening using liquid handler and cell imager. Also we can compare cell behavior between 2D and 3D system.

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Setting adequate culture conditions for screening
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We check and set adequate conditions (media, supplement, cell seeding number etc. ) for screening your library using recommended cancer cells.
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Drug screening
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We evaluate the drug effect to cancer cell by luminescence ATP assay.
* Drug concentration and experiments will be decided by counseling
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Observation of spheroid morphology change
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We take the picture of spheroid morphology for better understanding the drug action.
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OPTION: target gene (mRNA/protein) analysis
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If you want to know your target gene expression (mRNA/protein) and/or statement of phosphorylation status, we can check it.

Example Data

Drug screening (compare the effect between 2D and 3D condition)

We conducted drug screening: using 1330 chemical compounds in 2D and 3D condition. In 3D, most of compounds indicated higher resistance, and a few compounds indicate more effective than in 2D.

Drug effect to spheroid morphology

PRICE
US$ 55.00/well~