1. HOME
  2. Services
  3. screening of EMT inhibitor

Services

screening of EMT inhibitor

For customer who wants to explore candidate of anti cancer metastasis drug

We conduct EMT inhibitor screening with unique assay system. In this system, we use NCP for 3D cell culture, and Hypoxia Probe for quantifying spheroid morphology change that induced by EMT cytokine. This system is compatible with high-throughput screening using chemical library.

Item
Content
Item
Establishing culture condition
Content
We choose cell line and cytokine according to your needs, and establish adequate conditions (media, cell seeding number etc.) for the screening
Item
EMT inhibitor screening
Content
We conduct EMT inhibitor screening using the established assay protocol
Item
Checking cytotoxicity
Content
By luminescence ATP assay, we check cytotoxicity of chemical compounds. And we calculate the signal intensity effect of Hypoxia Probe.
Item
OPTION:
analysis of EMT marker gene expression
Content
We can validate the achieved hit compounds by evaluating the change of EMT marker gene expression

Example Data

EMT inhibitor screening

Using our assay system, some candidates of EMT inhibitor, include TGF-β receptor inhibitor, were screened from 1330 chemical compounds.

Validation: evaluation of expression change of EMT marker genes by qRT-PCR

E-Cadherin

N-Cadherin

Vimentin

zeb1

It is confirmed that these hit compounds have the EMT inhibitor activity by evaluating the expression alteration of EMT marker genes.

PRICE
US$ 90.00/well~

Example
Please download from here.