Establishment of 3D cell culture experimental condition
For customer who wants to establish 3D cell culture according to one’s experiments
We offer 3D cell culture protocol according to customer’s needs. At first, we compare and choose suitable 3D cell culture method from scaffold based methods i.e. NCP (ORGANOGENIX), Matrigel (Corning) or suspension based method i.e. Round bottom (Corning). We check the spheroid formation, proliferation, gene expression level (if needed).
Comparison of several 3D cell culture methods: HepG2 spheroid feature after 7 days culture
Comparison of several 3D cell culture methods: Growth curve of HepG2（ATP assay）
Visualization of hypoxic condition inside spheroids
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