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Establishment of 3D cell culture experimental condition

For customer who wants to establish 3D cell culture according to one’s experiments

We offer 3D cell culture protocol according to customer’s needs. At first, we compare and choose suitable 3D cell culture method from scaffold based methods i.e. NCP (ORGANOGENIX), Matrigel (Corning) or suspension based method i.e. Round bottom (Corning). We check the spheroid formation, proliferation, gene expression level (if needed).

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Spheroid formation
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We confirm spheroid size, number and morphology under various experimental conditions (media, cell seeding number, culture term) in each culture methods.
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Spheroid growth curve
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At optimized culture condition, we make growth curve by luminescence ATP assay. That will help us to determine the optimum doubling time and culture time.
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Spheroid size and cell-cell adhesion
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We observe hypoxic condition inside the spheroid using Hypoxia Probe. Because the degree of intra spheroid hypoxia correlate with spheroid size and cell-cell adhesion.
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Measuring gene expression
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If it is needed, we can check expressions level of some genes in 3D culture. For micro-array, we can prepare a large amount of mRNA

Example Data

Comparison of several 3D cell culture methods: HepG2 spheroid feature after 7 days culture

Comparison of several 3D cell culture methods: Growth curve of HepG2(ATP assay)

Visualization of hypoxic condition inside spheroids

PRICE
Please feel free to contact.

Example
Please download from here.