Introduction of NanoCulture Technologies
Micro-Patterned Surface for Scaffold
NanoCulture Plate (NCP) and NanoCulture Dish (NCD) are engineered with micro-patterned structure on a special plastic film on the well-bottom surface. This pattern act as a scaffold for cells, and support cells to form cell spheroids. This nano-scale structure control the minimum attachment of cells to the film and enhances cells migration and proliferation while cells form spheroids.
Nano-scale patterned well-bottom film
Structural color is observed, due to the interference of light by nano-scale pattern.
HeLa cell spheroid attaching to nano-scale structure of film
Several cells, constituting spheroid, contact the well-bottom film, grip and attach to nano-scale pattern using pseudopodium.
Data provided by: Prof. Hideo Namiki, Waseda University
Spheroid formation mechanism on NanoCulture Plate / Dish
Cells seeded on NCP/NCD grip and attach to the nano-scale pattern on culture surface. Cells start migrating randomly and dynamically because these do not adhere strongly to the base film due to micro-structure, unlike conventional monolayer cell culture. Cells contact and adhere to each other while migrating on the surface, and form spheroids accompanied by proliferation at the same time. Spheroid growth reaches a plateau as culture period progresses. These cellular behavior is common through the 3D cell culture system using hydrogel or any other gel based matrix.
Spheroid Formation Process of BT474 Cell Lines
SEM images of time-dependent morphological change of HeLa cells spheroid.
Individual cells of spheroid are distinguished at day 2. Shape of the cells became indistinct gradually, and spheroid is covered with EMC at day 6. Spheroid size grow up time-dependently.
Chronological change of spheroid size of HepG2 cells
Box plot of spheroid diameter obtained from HepG2 cells cultured for 7 days on NCP (96 well plate) at 10,000 cells/well. Spheroids keep growing as culture period progresses, while it reaches to a plateau in approximately one week.