Anti-Cancer Drug Screening
In vitro drug sensitivity studies using conventional monolayer culture (2D) differ substantially with the in vivo tumor. There are two major reasons for this difference.
First of all, the monolayer culture is quite an artificial cell culture condition depending on anchorage and finally, 2D cultures are inadequate representations of in vivo tumor microenviroments. This gap between in vitro and in vivo experiment is a huge barrier in anticancer drug development. Spheroid is expected to be a bridge between in vivo and in vitro, because tumor cells spheroids show a higher degree of morphological and functional similarity like in vivo.
Drug sensitivity difference between 2D and 3D
Approximately 1,300 compounds were treated with human A549 lung cancer cell line both in 2D and 3D conditions. Lowered drug sensitivity with 3D system compared to 2D are indicated in blue, and higher in red, and not significant difference in gray.
Compounds provided by: The University of Tokyo Open Innovation Center for Drug Discovery
Sensitivity difference against Trastuzumab between 2D and 3D
Human breast cancer cell line BT474 showed stronger sensitivity against Anti-HER2 antibody Trastuzumab (10 μg/mL). Viability was measured by ATP assay, DNA quantitation, and cell counting.