It is known that hepatocyte cells lose their original function, e.g. secreted albumin capacity, metabolic capacity in monolayer culture condition. Recently, it was reported that hepatocyte cell line and immortalized hepatocyte cells can retained their function under 3D culture condition. And it is also reported for primary hepatocyte cell culture under 3D condition.
Rat primary hepatocyte
Hepatocyte cells spheroid
Primary rat hepatocyte HepG2 and Fa2N-4 form spheroids on NCP.
Enhancement of immortalized human hepatocytes function
Higher level of albumin secretion or gene expression of CYP space’s under 3D culture condition compared to 2D culture condition.