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Differentiation Induction of Mesenchymal Stem Cell

Conventional 2D cell culture

3D cell culture on NCP

Differentiation of human mesenchymal stem cells UET-13 to adipocyte
Cultured human immortalized mesenchymal stem cell UET-13 in both 2D and 3D culture condition, and induced differentiation to adipocyte. By staining lipid-droplet with Oil red O, most of the cells were stained red, in case of 3D culture. Also, marker gene expressions showed quicker and stronger increase, compared to 2D culture, which indicates higher efficiency of differentiation into mature adipocyte, under 3D culture condition.
Data provided by: Dr. Hajime Okita of National Research Institute for Child Health and Development